Parvovirus Methods and Compositions for Killing Neoplastic Cells”

ID# 2003-2817
AAV2 ribbon diagram

Technology Summary

The limited ability of anti-neoplastic therapy to distinquish neoplastic from normal cells continues to be a primary hurdle in the treatment and irradiation of neoplastic, tumor or other cancerous cells. Adeno-associated virus (AAV)-based vectors have been shown to be nonpathogenic vectors with potential for cancer gene therapy. AAV is ubiquitous, non-pathogenic, anogenital virus with tumor suppressive properties, including against the development of cervical cancer. The subject invention covers a type of AAV which selectively mediates apoptosis as a mode of oncosuppression in HPV-infected cell lines. Normal, non-cancerous cells infected with the invention did not undergo apoptosis and displayed no cytopathic effects.

Application & Market Utility

The research results show that the invention can induce cell death of cervical cancer cells at all stages of carcinogenic progression: preneoplastic cervical intraepithelial neoplasia I up to cervical invasive carcinoma cells. Experimental results including Western blot analysis, Southern blot analysis, Hirt DNA extraction and DNA fragmentation analysis provided confirmation regarding these apoptosis conclusions.

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